Test principle

At which pregnancy weeks can Actim PROM test be used?

  • Actim PROM test can be used at any gestational age, in which there may be need for testing the leakage of amniotic fluid. IGFBP-1 levels rise rapidly in early pregnancy.

Is Actim PROM test affected by medications or lubricants?

  • No: several substances have been tested and demonstrated not to affect the test result.

Is Actim PROM test affected by shower products?

  • No: several substances have been tested and demonstrated not to affect the test result.



Sample handling

How is the sample handled?

  • The sample is taken with the polyester swab that is included in the test package:Actim PROM sample is taken from the vagina.
  • After the swab has absorbed the sample, it is transferred and extracted in the sample extraction solution. At this point, the sample is ready for testing.

Can the same sample be used for both Actim PROMand Actim Partus tests?

  • Separate samples need to be taken for the two tests. The sample for Actim Partus test must be taken from the cervix, while a vaginal sample is used for Actim PROM test.

  • The test requires at least 150 l of sample to perform correctly, and this may also limit the possibility to run two dipstick tests from the same sample tube.

Test procedure

Can the dipstick be left in the sample to develop?

  • The dipstick should be removed from the sample as soon as the liquid front becomes visible in the result window. Appearance of the liquid front in the result window indicates that enough sample has been absorbed by the dipstick.

  • If the dipstick is left in the sample, the dipstick goes on absorbing the sample which in turn may lead into uncontrollable flow.

Test results

How does blood contamination affect the test result?

  • The concentration of IGFBP-1 in amniotic fluid is 100-1000 times higher than in maternal serum, and even a very small amount of amniotic fluid will be detected by Actim PROM test.

  • The detection limit of the test has been set to 25 g/l in the extracted sample. The cut off has been optimized to a level where blood contamination is highly unlikely to affect the test result.

Do the results need to be read exactly at 5 minutes?

  • A positive result can be interpreted as soon as two blue lines become visible in the result window.

  • However, a negative test must be confirmed at 5 minutes. As the immunochromatography reaction continues to develop over time, the lines may become stronger or lines may appear in the result area. These lines must not be interpreted as positive as they indicate analyte levels below the detection limit of the test.

Should the intensity of the test line be compared to the intensity of the control line?

  • The result is interpreted by counting the number of lines in the result area. No attention should be paid to the relative intensities of the control and test lines.

What should be done if no control line appears?

  • The control line confirms correct performance of the test. If the control line does not appear, the test result is invalid and should be repeated with another dipstick.

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